Histology Core
The CBR-managed histology core is currently located in Bertelsmeyer 220 and features all necessary equipment for tissue sample processing and staining, including a Sakura Tissue-Tek VIP 5 tissue processor and a Leica CM1860 cryostat. All equipment is currently free to use for CBR members and their students.
All prospective users will be required to complete training before keycard access to the facility will be granted. Required training includes general laboratory safety training located at BSL-2 safety training (contact Michelle Bresnahan for training, Equipment use must also be scheduled in advance through the Histology Users - MST Outlook group calendar.
Please contact Anna Chernatynskaya or Katie Tooley for more information and for required training.
List of Equipment
Equipment manuals and SOPs are linked below each image.
Leica CM1860 Cryostat
Sakura Tissue-Tek VIP 5 Tissue Processor
Leica EG1150 H&C Tissue Embedder and Cold Plate
Leica RM2235 Microtome
Leica Autostainer XL
Olympus BX53F Fluorescent Microscope
User-Provided Supplies
The following items must be supplied by users/PIs. They will not be provided by the CBR.
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Blades for microtome and cryostat: Epredia HP35 Ultra microtome blades (please stick with this exact product, our microtome is set up for easy change-outs of these specific blades) - $289.09 for a pack of 50, Catalog #31-537-35, https://www.fishersci.com/shop/products/thermo-scientific-ultra-disposable-microtome-blades-2/3153735?searchHijack=true&searchTerm=thermo-scientific-ultra-disposable-microtome-blades-2&searchType=Rapid&matchedCatNo=3153735
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Camel hair brushes, small (links include what we use, but feel free to shop around, must be camel hair) - $70.75 for a pack of 12, Catalog #1910, https://www.fishersci.com/shop/products/cryotome-cryostat-accessories-camel-hair-brush/1910#?keyword=1910%20brush
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Camel hair brushes, large - $26.28 each, Catalog #03-661, https://www.fishersci.com/shop/products/fisherbrand-long-handled-camel-s-hair-brush/03661#?keyword=03661
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1 gallon of Fisher histological grade ethanol (only needed if you'll be processing more than 50 samples) - $113.10, Catalog #A405F-1GAL, https://www.fishersci.com/shop/products/ethanol-anhydrous-histological-fisher-chemical-3/A405F1GAL?searchHijack=true&searchTerm=ethanol-anhydrous-histological-fisher-chemical-3&searchType=Rapid&matchedCatNo=A405F1GAL
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Glass microscope slides (charged slides are best for tissue retention during staining) – Fisherbrand Superfrost Plus Microscope Slides, $47.66 for pack of 144 slides, Catalog # 22-034979, https://www.fishersci.com/shop/products/fisherbrand-superfrost-plus-stain-slides/22034979?searchHijack=true&searchTerm=fisherbrand-superfrost-plus-stain-slides&searchType=Rapid&matchedCatNo=22034979
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Glass cover slips (personal preference, but this is what we use) - Epredia Signature Series Cover Glass, $83.55 for a pack of 10 boxes, Catalog #22-050-232, https://www.fishersci.com/shop/products/signature-series-cover-glass-24-x-50mm/22050232#?keyword=22050232
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Microscope slide box (feel free to shop around, item linked is an example) - Fisherbrand Microscope Slide Box, 100 slots, $9.58, Catalog #03-446, https://www.fishersci.com/shop/products/fisherbrand-microscope-slide-boxes-numbered-slots-3/03446#?keyword=03-446
Additional Resources
- Leica Knowledge Pathway: https://www.leicabiosystems.com/us/knowledge-pathway/
- Histological Techniques: https://mmegias.webs.uvigo.es/02-english/6-tecnicas/1-introduccion.php
- Pathology Innovations Cryosectioning Techniques: https://pathology-innovations.squarespace.com/
Contact Us
Anna Chernatynskaya
Lab Manager
573-341-4029 | chernatynskayaa@mst.edu |
Bertelsmeyer 218
Katie Tooley
Lab Manager
Bertelsmeyer 218