Overview
The CBR-managed histology core features all necessary equipment for tissue sample processing and staining, including a Sakura Tissue-Tek VIP 5 tissue processor and a Leica CM1860 cryostat.
Location: Bertelsmeyer 220
Cost: A list of fees can be found in the CBR Equipment and Services Usage Rates document.
Note: A fee waiver application is available for PIs without current external funding. The application may be completed and submitted to the CBR director prior to equipment use.
Required Training
All prospective users will be required to complete training before keycard access to the facility will be granted. Please contact Anna Chernatynskaya or Katie Tooley for more information and for required training.
Required training includes
- General laboratory safety training located under the EHS Training Index
- BSL-2 safety training (contact Michelle Bresnahan for training, mem@mst.edu)
- Training for all equipment
Schedule Equipment
Equipment use must also be scheduled in advance through the Histology Users - S&T Outlook group calendar.
Histology Equipment
Equipment manuals and SOPs are linked below each image.
Leica CM1860 Cryostat
Sakura Tissue-Tek VIP 5 Tissue Processor
Leica EG1150 H&C Tissue Embedder and Cold Plate
Leica RM2235 Microtome
Leica Autostainer XL
Olympus BX53F Fluorescent Microscope
User-Provided Supplies
The following items must be supplied by all PIs utilizing the histology equipment due to high costs or variable use.
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Blades for microtome and cryostat: Epredia HP35 Ultra microtome blades (please stick with this exact product, our microtome is set up for easy change-outs of these specific blades) - $289.09 for a pack of 50, Catalog #31-537-35
- Microscope slide box (feel free to shop around, item linked is an example) - Fisherbrand Microscope Slide Box, 100 slots, $9.58, Catalog #03-446
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Glass microscope slides (charged slides are best for tissue retention during staining) – Fisherbrand Superfrost Plus Microscope Slides, $47.66 for pack of 144 slides, Catalog # 22-034979
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Glass cover slips (personal preference, but this is what we use) - Epredia Signature Series Cover Glass, $83.55 for a pack of 10 boxes, Catalog #22-050-232
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Any reagents and supplies required for non-H&E staining
The following item must be supplied by the PI if a histology fee waiver form has been approved or if use of the CBR-provided supplies is excessive.
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1 gallon of Fisher histological grade ethanol (only needed if you'll be processing more than 50 samples) - $113.10, Catalog #A405F-1GAL
The following items are recommended to be supplied by the PI, but are not required.
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Camel hair brushes, small (links include what we use, but feel free to shop around, must be camel hair) - $70.75 for a pack of 12, Catalog #1910
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Camel hair brushes, large - $26.28 each, Catalog #03-661
Additional Resources
Contact Us
Anna Chernatynskaya
Lab Manager
573-341-4029 | chernatynskayaa@mst.edu |
Bertelsmeyer 218
Katie Tooley
Lab Manager
Bertelsmeyer 218